Regulatory

Part:BBa_K4613006:Design

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)


Ptra


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reference

  1. White CE, Winans SC. Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription. Mol Microbiol. 2005 Mar;55(5):1473-86. doi: 10.1111/j.1365-2958.2004.04482.x. PMID: 15720554.

Fuqua C, Winans SC. Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J Bacteriol. 1996 Jan;178(2):435-40. doi: 10.1128/jb.178.2.435-440.1996. PMID: 8550463; PMCID: PMC177675.


Source

Originally from Agrobacterium tumefaciens R10. In this part, the RBS is from the LuxI/LuxR quorum sensing system.

References